Part:BBa_K1053014:Design

TR(12)-HHR-taR12y

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 159
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 159
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 159
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The region recognizing TR-HHR in taRNA is sequestrated in HHR's extended stem I.

Source

This is composed of BBa_K1053000(taRNA responsive Hammerhead Ribozyme(TR-HHR) with RBS) & BBa_K1053010(PBAD-taR12y-DT)